Antibacterial activity of the phlorotannins dieckol and phlorofucofuroeckol-A from Ecklonia cava against Propionibacterium acnes

  • Jae-Suk Choi RIS Center, Industry-Academic Cooperation Foundation, Silla University
  • Kyungyong Lee Gijang Local Products Co. Ltd.
  • Bo-Bae Lee RIS Center, Industry-Academic Cooperation Foundation, Silla University.
  • Yang-Chun Kim Gijang Local Products Co. Ltd.
  • Young Dae Kim East Sea Fisheries Research Institute, National Fisheries Research & Development Institute.
  • Yong-Ki Hong Department of Biotechnology, Pukyong National University.
  • Kwang Keun Cho Department of Animal Resources Technology, Gyeongnam National University of Science and Technology.
  • In Soon Choi RIS Center, Industry-Academic Cooperation Foundation, Depertment of Biological Science, Silla University.
keywords: dieckol, minimum inhibitory concentration, natural additives, phlorofucofuroeckol-A, phlorotannins

Abstract

Methanol extract of the marine brown alga Ecklonia cava has been shown to exhibit antimicrobial activity against Propionibacterium acnes. To identify the compounds conferring this antimicrobial activity, methanol extract was fractionated using silica-gel column chromatography by increasing solvent polarity, and the antimicrobial activities of each fraction were determined in terms of the minimum inhibitory concentration. Hexane, diethyl ether, acetone, ethyl acetate, acetonitrile, methanol, and water fractions were generated, with the acetone fraction exhibiting the greatest antibacterial activity (minimum inhibitory concentration = 156 ?g/mL). The acetone fraction was further puri? ed by Sephadex LH-20 column chromatography and reverse-phase HPLC using an Alltima C18 column with an H2O-MeOH linear gradient from 30 to 100%. Two peaks were eluted at 18.1 (P1) and 23.8 (P2) min, both of which exhibited marked antibacterial activity (minimum inhibitory concentration = 39 ?g/mL for both). The structures of these compounds were determined on the basis of 1H and 13C nuclear magnetic resonance data and, through comparison with published data, can be phloroglucinol derivatives dieckol (P1) and phlorofucofuroeckol-A (P2). These results suggest that phlorotannins derived from E. cava have antimicrobial activity against P. acnes, and so may be useful as natural additives in antiacne cosmetics and pharmaceutical products.

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Antibacterial activity of the phlorotannins dieckol and phlorofucofuroeckol-A from Ecklonia cava against Propionibacterium acnes
Published
2014-08-14
How to Cite
Choi, J.-S., Lee, K., Lee, B.-B., Kim, Y.-C., Kim, Y. D., Hong, Y.-K., Cho, K. K., & Choi, I. S. (2014). Antibacterial activity of the phlorotannins dieckol and phlorofucofuroeckol-A from Ecklonia cava against Propionibacterium acnes. Botanical Sciences, 92(3), 425-431. https://doi.org/10.17129/botsci.102
Section
PHYTOCHEMISTRY / FITOQUÍMICA